We are still open for nursery plant sales, and look forward to your live root and plant orders. Antimicrobial activity of polyphenols and alkaloids in. Plant tissue homogenization and extract preparation. Chemical constituents and pharmacological properties of. Leaves, seeds and roots contain the alkaloid daturine. Antimicrobial activity seed oil datura metel jebtm authors info r. These dried seeds were pulverized using sterile laboratory mortar and. The antibacterial activity of the methanol extracts of the aerial parts of the datura innoxia and datura stramonium was investigated. Phytochemical screening and antimicrobial assessment of. Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery.
Datura stramonium, callus induction, antibacterial activity, antifungal activity, phytochemical analysis. Phytochemical and antimicrobial property of datura stramonium. Zoology department, samrat prithviraj chauhan govt. Analysis of alkaloid phytochemical compounds in the. Various parts of datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against gramnegative bacteria escherichia coli and salmonella typhi and grampositive bacteria bacillus cereus, bacillus subtilis and staphylococcus aureus. Antimicrobial activities of methanolic extract of datura stramonium seeds material and methods. Its extracts show significant antimicrobial activity against staphylococcus aureus, proteusvulgaris, pseudomonasaeruginosa, escherichia coli, aspergillus niger and fusariumspecies. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of 1. The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of adhatoda vasica, azadirachta indica and datura stramonium leaf extract. Joshi 11 23 1 2 3 department of chemistry, graphic era university, dehradun248 002, india department of biotechnology, doon p. Home shop datura, jimson weed datura stramonium, packet of 50 seeds, organic we at strictly medicinal seeds are dedicated to providing medicinal plants to the people. Antimicrobial activity of datura innoxia and datura stramonium.
Alkaloids, antibacterial activity, datura stramonium, gas chromatographymass specroscopy. Antibacterial compounds in different species of datura a. Datura, jimson weed datura stramonium, packet of 50. Little or no antibacterial activity was found against escherichia coli and pseudomonas aeruginosa. Comparative bacterial inhibition by bioactive extracts from datura. Antimicrobial activity of datura innoxia and datura. Abstract datura stramonium is a plant of medicinal plant family solananceae. Ethanolic extract showed more antibacterial activity than the aqueous extract. Antimicrobial activity of some common indian medicinal plants against some selective human pathogen h. Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas. Its extracts show significant antimicrobial activity against staphylococcus aureus, proteus vulgaris, pseudomonas aeruginosa, escherichia coli, aspergillus niger and fusarium species 21. Antioxidant and antimicrobial properties of seed oil of.
Antimicrobial activity of cotton and silk fabrics dyed. Besides its hallucinogen activity, datura reported to have antimicrobial activity rajesh and sharma, 2002. Pdf antimicrobial investigation of datura stramonium leaf. Chemical tests and spectrophotometric methods were used for antioxidant activity. Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas aeruginosa, agrobacterium tumefaciens and klebsiella. Datura stramonium is commonly known as jimson weed and is widely used in phytomedicine to treat epilepsy, skin ulcers, etc as well as to kill the microbes associated with wound lesions. Datura stramonium extract, zone of inhibition, antibacterial activity, antifungal activity, agar well diffusion method, minimum inhibitory concentration. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university, vellore632 014, tamil nadu, india. Antimicrobial activity of some common indian medicinal. Antibacterial and antifungal activity of different extracts of datura. Phytochemical screening and antimicrobial assessment of abutilon. Datura stramonium could be potential sources of chemotherapeutic agents. Nematicidal activity of aqueous leaf extracts of datura. Aerial part mainly stem and bark of datura stramonium s aqueous and ethanolic extract opposite to eschericia coli.
In this study, crude extracts from fungal endophytes isolated from datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. The general results observed consider the plant as natural source of antioxidants and phytochemical quality for. College of arts, science and commerce, chembur, mumbai 400071, india. The secondary metabolites of datura stramonium are highly effective against different types of diseases such as antdiabetic, antiviral, etc. Datura stramonium, crude extract, phytochemical screening, antimicrobial activity, paper disk diffusion method. The bacterium streptococcus mutants was resistant to methanol extract while the fungus candida albicans was resistant to methanol and acetone extract. Datura stramonium solanaceae is an important medicinal plant from which tropane alkaloids, amino acids, tannin, phytic acids, carbohydrates have been isolated. Test organism the microorganism used for this study, bacterium. The dried seeds of datura stramonium which were obtained from seed pods of datura plant.
An extract of datura stramonium was screened for phytochemical analysis and antimicrobial assay against different bacterial species. Introduction datura species can be found throughout the world, except the colder or arctic regions, it grows wild in all of the worlds warm and moderate region and it is very distributed in the temperate and tropical areas 18. The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of datura metel, datura innoxia and brugmansia suaveolens. Pdf antimicrobial investigation of datura stramonium. Little or no antibacterial activity was found against escherichia coli and. Datura stramonium is a natural source of antioxidants and phytochemical having antimicrobial activities. In vitro and in vivo gcms profile and antimicrobial activity of phytosterols of datura stramonium. Datura stramonium linn solanaceae grows as a wasteland weed 4, 5 mostly in the tropical and temperate areas of the world. It is common in the waste land, fields and gardens in baghdad district figure 1. The antibacterial activity reveals that the methanolic extract of datura stramonium seeds is highly active against both gram positive and gram.
Joshi and kaur 20, worked on antimicrobial activity of ethanol, methanol and aqueous extract of d. Richa bhardwaj, ankita yadav, pallavi sharma and ram avatar sharma, 2014. Datura stramonium was examined for its antimicrobial activity on some oral pathogens using methanol, chloroform, acetone and water as extractants. Datura stramonium plants on drug resistant pathogens isolated from clinical specimens dr. Comparative antimicrobial activity and phytochemical analysis of datura stramonium l. Nagesh malik1, samreen ahmed2 1,2 department of microbiology, v. All datura plants contain tropane alkaloid such as hyoscyamine, scopolamine and atropine in the seeds and the flowers. Little or no antimicrobial activity was found against escherichia coli and psuedomonas aeruginosa. Abstract the phytochemical analysis and antimicrobial activities of in vitro grown callus and in d. Datura stramonium and phytolacca dodecandra in different solvents.
In vitro evaluation of datura innoxia thornapple for. Extraction and purification of antimicrobial proteins from. Paramedical college, dehradun248 991, india department of environmental science, graphic era university. The extraction was carried out using trishcl buffer prepared from 50m tris and 0. Comparative antimicrobial activity and phytochemical analysis of. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts. Evaluation of acute and subacute toxicity of alkaloids. The aim of this study was to assess the compounds of alkaloids extracts from the leaves of datura stramonium, which can be the basis for the synthesis of new antibiotics. This study is conducted to investigate the antimicrobial property of proteins present in the seeds of datura stramonium. Phytochemical and antimicrobial property of datura stramonium 140 pestle.
Datura stramonium is an annual herbaceous plant of the solanaceae family. Callus induction and antimicrobial activities of callus. Introduction datura stramonium is an annual herb, with stem erect and spreading branches above. Dried plant leaves of datura stramonium, carica papaya and fruits of piper nigrum. Antibacterial, phytochemical and antioxidant activities of.
Pdf analysis of alkaloid phytochemical compounds in the. Its diverse biological activities include antiasthmatic, antibacterial, antifungal, antiinflammatory, larvacidal. The results of agar well diffusion assay indicated that the pattern of inhibition depends largely. Phytochemical analysis, antioxidant and in vivo biological. The aim of the present study was to investigate the antimicrobial activity and phytochemical screening. Antimicrobial activity of cotton and silk fabrics dyed with datura stramonium jimson weed plant leaf extracts alex musinguzi department of manufacturing, industrial and textile engineering, school of engineering, p. The main objective of this study was to evaluate the phytochemical composition, antioxidant, antifungal and insecticidal activities of aqueous and methanolic extracts. Comparative bacterial inhibition by bioactive extracts. Datura stramonium the antimicrobial activity of the aqueous and ethanolic extract of the stembark of datura stramonium was investigation against staphylococcus aureus, salmonella typhi, shigella spp, eschericia coli, klebsiella pneumonia and neisseria gonorrhea. The present study was conducted to identify the preliminary phytochemical identification and antimicrobial investigation of leaves extracts of datura stramonium linn collected from eastern ethiopia.
The solvent used for extract preparation was methanol. The fine powder were collected into a sterile aluminum foil and kept in a cool dry place till further use. Datura stramonium is traditionally used to cure different human diseases including in skin disorder, ear pain, cough, fever, burns, and asthma in ethiopia. Natural dyes and its antimicrobial effect smita bhuyan, dr. Antimicrobial property of datura leaf extract against. The acceptance of traditional medicines as an alternative form of health care has led researchers to investigate the antimicrobial activity of medicinal plants 2, 3. Antibacterial activity of the plant extract and determination of minimum inhibitory concentration. Antimicrobial activity of carica papaya, piper nigrum and. In vitro and in vivo gcms profile and antimicrobial. Manjunath department of biochemistry, central college campus, bangalore university. Pdf antimicrobial property of datura leaf extract against methicillin. Fungal endophytes were isolated from surface sterilized aerial parts of d.
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